Of this, the CD44 has been analyzed. CD44, a type of cell-surface glycoprotein and E-selectin ligand, plays a critical role in cell-cell adhesion, cell interactions and cell migration. In 12 most common isoforms of CD44, there are 4 isoforms that are CD44s, CD44v3, CD44v4, CD44v6 which are mainly expressed in breast carcinoma cells. CD44 cells bind to the hyaluronan and fibrin (ogen). It has been found that the alternative splicing is the reasons for many differential isoforms . In this, inserting various forms of exons into the extracellular membrane’s proximal region generates the variant isoforms. For this purpose, the force spectroscopy is used to analyze the biophysical and molecular requriements. CD44 is binding to the hyaluronan and fibrin (ogen) is analyzed by combining force spectroscopy with the biochemical interventions.
This is the explanation of the overall metastasis action of the cells. However, these are only the overall mechanism to understand the impact of the actual breast cancer metastasis. It has been found that they use cell lines. Immortalized cell lines are developed in order to develop an enhanced understanding of the cellular functions. It has been found that there are a number of commercially developed cell lines for the purpose of its biotechnological and diagnostic functions.
It has been further understood from analyzing the cell lines. Glycosylation process plays an important role in the metastasis function. In this particular analysis, the role of the distinct glycosylation within the E-selectin ligand has been probed. E-selectin ligand is found in the endothelial layer and is important for the adhesion mechanism. This has been probed in detail in the following.