戴顿大学论文代写:一种新的抗细菌大肠杆菌素的发现
戴顿大学论文代写:一种新的抗细菌大肠杆菌素的发现
大肠杆菌DH5α成功分离了，证明了他们能够维持并杀死其他E大肠杆菌菌株。TY培养基营养肉汤是一致的胰蛋白胨8，酵母膏5，5氯化钠而营养琼脂包容一层在基地0.5%，蛋白胨0.3%的酵母提取物，氯化钠0.5%与调整pH值至中性沿最顶端的层。为了测试的敏感性的蛋白酶抑制剂，此外，胰蛋白酶（0.1厘）是在顶部（布劳恩等1994）的软琼脂层的。所有这些过程，不同的技术被用来以获得的结果在尽可能最好的形式准确，这是为什么滞留大肠杆菌基因分离产生大肠杆菌素所需测试描述准确的结果。
进一步，它是预期的细胞可以产生一个新的大肠杆菌素，但它是测试发现，DH5α大肠杆菌细胞不会导致对生产colicins但转化质粒后，他们获得了大肠杆菌细胞的杀伤能力。
这些结果的影响是巨大的，具有很大的潜力。发现一个特定的大肠杆菌DH5α菌株能利用该质粒援助产生大肠杆菌素杀死其他品系的大肠杆菌。它所需要的自然环境介质是人类的肠道菌群。经实验分析，分离菌株产生的毒素在大肠杆菌素的形成，造成另一株大肠杆菌，有趣的是它是不是对自己有害的（布劳恩等，1994）。
戴顿大学论文代写:一种新的抗细菌大肠杆菌素的发现
Successful isolation of E.Coli DH5 Alpha was done and proved that they were able to sustain and kill the other E-.Coli strains. TY medium nutrient broth was consistent with Tryptone 8, 5 yeast extract, 5 sodium chloride whereas the nutrient agar was inclusive of a layer in the base with 0.5 per cent, peptone with 0.3 percent from extract of yeast, Sodium Chloride to be 0.5 per cent with adjustment of PH to neutrality along with a top most layer. In order to test the sensitivity of proteinase within the agents of inhibition, addition of trypsin (0.1 per cent) was done to the soft agar layer at the top (Braun et al 1994). For all these processes, different techniques were used in order to obtain results in the best possible form with accuracy and this is why the stranded Ecoli genes were separated to produce a colicin which required testing to depict accurate results.
Further, it was expected that the cells may be producing a new colicin but it was tested to find that DH5 alpha E coli cell does not lead towards production of colicins but after being converted with the plasmid, they gained the capability of killing E-coli cells.
The implications of these results are huge with a lot of potential. It was discovered that a certain strain of E.Coli DH5 Alpha was able to kill other strains of E-coli by producing colicin with the aid of the plasmid. The natural environmental medium where it takes place is in the gut flora of human. It was found from experimental analysis that the isolated strain produced toxins in the form of colicin, which killed the other strains of E.Coli interestingly it was not harmful on themselves (Braun et al 1994).
